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KMID : 0359320010410010051
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Korean Journal of Veterinary Research
2001 Volume.41 No. 1 p.51 ~ p.57
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Development of ELISA for Brucella abortus RB51
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김옥경/Kim, Ok Kyung
조동희/정석찬/정병열/조성근/허문/Cho, Dong Hee/Jung, Suk Chan/Jung, Byeong Yeal/Cho, Seong Kun/Her, Moon
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Abstract
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A procedure for extraction and purification of 8 kDa antigen of Brucella abortus RB51 was developed. Bacteria heat inactivated at 60℃, 30 min was extracted by 1% sarcosine and followed by fluid pressure liquid gel filtration chromatography of 2 series, Superose 12 HR 10/30 and Sephacryl S-100. There was produced 71.46 ㎍/g(wet) of 8 kDa antigen, and it resisted 1% trypsin, solved 1% triton X-100 higher than distilled water and inactivated 0.1% proteinase K. These results show that 8 kDa antigen may be a lipoprotein existed cell surface of B. abortus RB51. Also, we developed ELISA using purified 8 kDa surface antigen of Brucella abortus RB51 strain, its specificity and sensitivity was 95.0%, 98.6%, respectively. As compared with dot-blot assay using whole cell and ELISA using 8 kDa antigen, its correlation was 93.5%.
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